TY  - JOUR
TI  - TBX1, a DiGeorge syndrome candidate gene, is inhibited by retinoic acid
AU  - Zhang, Lifeng
AU  - Zhong, Tao
AU  - Wang, Yuexiang
AU  - Jiang, Qiu
AU  - Song, Houyan
AU  - Gui, Yonghao
T2  - The International Journal of Developmental Biology
AB  - Both retinoic acid (RA) and Tbx1 are definitively indispensable for the development of the pharyngeal arches. The defects produced by a loss of Tbx1 highly resemble those induced by hyper- and hypo- RA. Based on these similarities, the effects of RA on Tbx1 expression pattern were explored during pharyngeal arch development in zebrafish. Whole-mount in situ hybridization and real-time quantitative PCR were used. Zebrafish embryos were treated with 5x10-8mol/L and 10-7mol/L RA at 12.5 hours post fertilization for 1.5 hours, respectively. Whole-mount in situ hybridization showed that Tbx1 was expressed in the cardiac region, pharyngeal arch and otic vesicle between 24 hpf and 72 hpf in zebrafish. Tbx1 expression was obviously reduced, even lost, in the pharyngeal arch and outflow tract in RA treated groups. Real-time quantitative PCR analysis showed that Tbx1 expression rose to a peak level at 36 hpf in wild type group. Repression of Tbx1 expression was most evident at 36 hpf, 24 hours after RA treatment. 10-7 mol/L RA caused a more severe effect on the Tbx1 expression level than 5x10-8mol/L RA.The results suggested that RA could produce an altered Tbx1 expression pattern in zebrafish. In addition, RA could repress Tbx1 expression in a dose-dependant manner.
PY  - 2006
DO  - 10.1387/ijdb.052036lz
VL  - 50
IS  - 1
SP  - 55
EP  - 61
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/052036lz
Y2  - 2024/05/06/00:20:01
ER  -